15522676233
15522676233
技術(shù)文章
更新時(shí)間：2022-11-24
點(diǎn)擊次數(shù)：1432
PEI又稱為線性化聚乙烯亞胺，是人工合成的含有高密度正電荷的有機(jī)大分子陽(yáng)離子聚合物。PEI與DNA通過(guò)正負(fù)電荷的相互吸引形成復(fù)合物，這些復(fù)合物很容易通過(guò)內(nèi)吞作用進(jìn)入細(xì)胞。PEI-DNA復(fù)合物能很好地抵御溶酶體的降解作用，有效提高轉(zhuǎn)染效率。PEI可作用于大至135000個(gè)核苷酸的質(zhì)粒，所以較大的質(zhì)粒也可以成功轉(zhuǎn)染。目前市面上PEI轉(zhuǎn)染試劑有很多種，Polysciences的PEI線性轉(zhuǎn)染試劑適用于多種真核細(xì)胞，用途廣泛。
一、Polysciences線性PEI轉(zhuǎn)染試劑(PEI 25K)特性：
貨號(hào)：23966-1
分子式：(CH2CH2NH)n
分子量：25000
外觀：白色至黃色粉末
熔點(diǎn)：73-75℃
溶解性：溶于：熱水、低pH值的冷水、甲醇和乙醇；
不溶于：苯、YI醚和丙酮。
二、Polysciences聚乙烯亞胺轉(zhuǎn)染試劑(PEI 25K)的應(yīng)用：
| 遞送物質(zhì) | 轉(zhuǎn)染細(xì)胞類(lèi)型 | 轉(zhuǎn)染細(xì)胞舉例（包括但不限于） |
| DNA | 貼壁/懸浮細(xì)胞 | HEK293 |
| CHO | ||
| HELA | ||
| SF9昆蟲(chóng)細(xì)胞 | ||
| SF21昆蟲(chóng)細(xì)胞等真核細(xì)胞系 |
(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9. IF：19.160
試劑：PEI 25K；應(yīng)用：DNA轉(zhuǎn)染人胚腎293S細(xì)胞。
(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54. IF：10.633
試劑：PEI 25K；應(yīng)用：質(zhì)粒DNA轉(zhuǎn)染小鼠骨髓間充質(zhì)干細(xì)胞（D1）。
(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020. IF：6.630
試劑：PEI 25K；應(yīng)用：包裝質(zhì)粒pCMVdelta8.9、包膜質(zhì)粒VSV-G和PD-1-GFP、PD-L1-GFP或空GFP sFFV轉(zhuǎn)移質(zhì)粒轉(zhuǎn)染HEK293T細(xì)胞
(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276. IF：4.395
試劑：PEI 25K；應(yīng)用：轉(zhuǎn)染HEK293T細(xì)胞。
(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739. IF:4.345
試劑：PEI 25K(23966-1)；應(yīng)用：質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞。
(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373. IF：3.602
試劑：PEI 25K(23966-1)；應(yīng)用：用shRNA-PLKO.1質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞。
(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132. IF：3.595
試劑：PEI 25K；應(yīng)用：質(zhì)粒DNA轉(zhuǎn)染懸浮生長(zhǎng)的CHO DG44細(xì)胞。
(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.
試劑：PEI 25K；應(yīng)用：用DNA轉(zhuǎn)染HEK293T細(xì)胞。
(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.
試劑：PEI 25K；應(yīng)用：將編碼fLuc基因的DNA遞送至小鼠。
(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.
試劑：PEI MAX(24765)；應(yīng)用：用質(zhì)粒DNA轉(zhuǎn)染ET801載體—幼倉(cāng)鼠腎來(lái)源細(xì)胞。
北京百奧創(chuàng)新科技有限公司提供多款Polysciences轉(zhuǎn)染試劑，具體信息請(qǐng)咨詢百奧創(chuàng)新客服。(Polysciences PEI 25K 23966-1現(xiàn)貨打折，*！機(jī)會(huì)有限，不要錯(cuò)過(guò)！)
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